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Cloning and Expression
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Stem Cell Culture Media
Differentiation Media
3T3-L1 Differentiation Medium, 100ml
3T3-L1 Differentiation Medium, 100ml from Zen-Bio, Inc.
Be the first to write a review!
Citations:
(18)
Supplier Page
Supplier Page from
Zen-Bio, Inc. for
3T3-L1 Differentiation Medium, 100ml
Product Specs
Company Info
Citations
Reviews
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Product Specs
Item
3T3-L1 Differentiation Medium, 100ml
Company
Zen-Bio, Inc.
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
DM-2-L1
Quantity
100ml
Temperature
4C
Type
MEDIA
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Zen-Bio, Inc.
3920 South Alston Ave
Durham
,
North Carolina
27713
United States
Phone
: (866)234-7673 or (919)547-0692
(919)547-0693
Company Profile
Website:
http://www.zen-bio.com
Citations (18)
(1)
Anti-Adipogenic Activity of Secondary Metabolites Isolated from Smilax sieboldii Miq. on 3T3-L1 Adipocytes
International Journal of Molecular Sciences
May 16, 2023
Yeon Woo Jung, Jung A Lee, Ji Eun Lee, Hanna Cha, Yun-Hyeok Choi, Wonsik Jeong, Chun Whan Choi, Joa Sub Oh, Eun-Kyung Ahn, Seong Su Hong
media [3T3-L1 Differentiation Medium (3-isobutyl-1-methylxanthine, dexamethasone, and insulin (MDI));
DM-2-L1
,
Zenbio
] was performed. The initiation medium was changed on day two with the 3T3-L1 Adipocyte Medium
(2)
Investigation on the Biological Safety and Activity of a Gluconolactone-Based Lotion for Dermocosmetic Application
Pharmaceuticals (Basel)
April 27, 2023
Nicola Zerbinati, Serena Di Francesco, Maria Chiara Capillo, Cristina Maccario, Giorgio Stabile, Hassan Galadari, Raffaele Rauso, Sabrina Sommatis, Roberto Mocchi
USA); two days after reaching confluence, the culture medium was switched to differentiation medium (
DM-2-L1
,
Zen-Bio
, Durham, NC, USA) for 3 days at 37 °C. Then, cells were maintained in maintenance medium (
AM-1-L1
,
(3)
Tumor-derived IL-6 and trans-signaling among tumor, fat, and muscle mediate pancreatic cancer cachexia
The Journal of experimental medicine
June 7, 2021
Rupert JE, Narasimhan A, Jengelley DHA, Jiang Y, Liu J, Au E, Silverman LM, Sandusky G, Bonetto A, Cao S,
et al
.
ensure growth arrest, refreshing the preadipocyte media every 48 h. Cells were then washed with PBS, DM (
DM-2-L1
;
Zen Bio
) was added to the wells, and the cells were placed back into the incubator. Cells were cultured
(4)
GRIM19 Impedes Obesity by Regulating Inflammatory White Fat Browning and Promoting Th17/Treg Balance
Cells
January 15, 2021
Jhun J, Woo JS, Lee SH, Jeong JH, Jung K, Hur W, Lee SY, Ryu JY, Moon YM, Jung YJ,
et al
.
Subsequently, the cells were incubated in an appropriate volume of
3T3-L1 cell
differentiation medium (
DM2-L1
,
ZenBio
) for 3 days, followed by
incubation
with
3T3-L1
adipocyte
maintenance medium (
AM-1-L1
,
ZenBio
).
(5)
Screening and Identification of an Anti-inflammatory and Anti-adipogenic Constituent from
Ligularia taquetii
Nakai
Natural Product Communications
January 1, 2020
Sungchan Jang, Min-Seon Kim, Taejin Park, Ji H. Sim, Seung-Young Kim
of LTE and CGA, the culture medium was then replaced with 3T3-L1 Differentiation Medium (Cat. no.
DM-2-L1
,
ZenBio
, Research Triangle Park, United States) containing LTE and a 0.1% v/v DMSO vehicle. After 72
(6)
Biphasic Effect of Sildenafil on Energy Sensing is Mediated by Phosphodiesterases 2 and 3 in Adipocytes and Hepatocytes
International Journal of Molecular Sciences
June 19, 2019
Jheelam Banerjee, Antje Bruckbauer, Teresa Thorpe, Michael B. Zemel
air. Confluent pre-
adipocytes
were induced to differentiate with a standard differentiation medium (
DM2-L1
,
Zen-Bio Inc
., Research Triangle Park, NC, USA). Pre-
adipocytes
were maintained in this differentiation
(7)
Adipocyte-Derived Lipids Mediate Melanoma Progression via FATP Proteins
Cancer Discovery
August 1, 2018
Maomao Zhang et al.
no.
PM-1-L1
). 2 days post confluency, media was changed to differentiation medium (
ZenBio
, Cat no.
DM-2-L1
) and incubated for 5 days. Then, cells were maintained in their adipocytic, differentiated state with
(8)
MicroRNA-30 modulates metabolic inflammation by regulating Notch signaling in adipose tissue macrophages
International journal of obesity (2005)
June 1, 2018
Miranda K, Yang X, Bam M, Murphy EA, Nagarkatti PS, Nagarkatti M.
medium, differentiation medium, and
adipocyte
maintenance medium were also used (
Zen-Bio
,
PM-1-L1
,
DM-2-L1
,
AM-1-L1
). Conditioned medium was collected between days 7 and 14 post-differentiation, 0.22um filtered,
(9)
The Effect of Pericellular Oxygen Levels on Proteomic Profile and Lipogenesis in 3T3-L1 Differentiated Preadipocytes Cultured on Gas-Permeable Cultureware
PLOS ONE
March 29, 2016
Martin Weiszenstein, Nela Pavlikova, Moustafa Elkalaf, Petr Halada, Ondrej Seda, Jan Trnka, Jan Kovar, Jan Polak
Proteomic Profile on Gas-Permeable Cultureware culture medium was changed to differentiation medium (
DM2-L1
,
Zen-Bio Inc
., NC, USA) and cells were placed in sealed plastic chambers—modular incubators (Billups-Rothenberg, CA,
(10)
Role of Micro RNA-205 in Promoting Visceral Adiposity of NZ10 Mice with Polygenic Susceptibility for Type 2 Diabetes
Journal of Diabetes & Metabolism
December 15, 2015
Nikhil Adi, Jennipher Adi, Liliana Cesar, Paul Kurlansky, Arthur Agatston, Keith A Webster
to 100% confluency and after 2 days the media was replaced with adipogenic differentiation medium (
DM-2-L1
,
Zen-Bio
). After 3 days the
DM-2-L1
media was replaced by
adipocyte
maintenance medium (
AM-1-L1
,
Zen-Bio
).
(11)
Interaction between leucine and phosphodiesterase 5 inhibition in modulating insulin sensitivity and lipid metabolism
Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy
May 23, 2015
Michael Zemel, Fenfen Fu, Fenfen Bruckbauer, Antje Bruckbauer, Qiang Cao, Rui Wu, Hang Shi, Bingzhong Xue, Michael B Zemel
air. Confluent preadipocytes were induced to differentiate with a standard differentiation medium (
DM2-L1
;
Zen-Bio Inc
., Research Triangle Park, NC, USA). Preadipocytes were maintained in this differentiation
(12)
Macrophage Elastase Suppresses White Adipose Tissue Expansion with Cigarette Smoking
American Journal of Respiratory Cell and Molecular Biology
December 1, 2014
Takao Tsuji, Neil J. Kelly, Saeko Takahashi, Adriana S. Leme, A McGarry McGarry Houghton, Steven D. Shapiro
obtained from Zen-Bio, Inc. (Durham, NC). Cells were incubated for 2 days in
PM-1-L1
(
Zen-Bio
), moved to
DM-2-L1
(
Zen-Bio
), and incubated for 3 days. Differentiated
adipocytes
were maintained in
AM-1-L1
(
Zen-Bio
).
(13)
Capsaicin Induces “Brite” Phenotype in Differentiating 3T3-L1 Preadipocytes
PLoS ONE
July 29, 2014
Ritesh K. Baboota, Dhirendra P. Singh, Siddhartha M. Sarma, Jaspreet Kaur, Rajat Sandhir, Ravneet K. Boparai, Kanthi K. Kondepudi, Mahendra Bishnoi
Triangle Park, NC). Dulbecco's modified Eagle's medium (DMEM),
3T3-L1
adipocyte
differentiation media (
DM-2-L1
) and
3T3-L1
adipocyte
maintenance media (
AM-1-L1
) were obtained from
Zenbio
Inc. (Research Triangle
(14)
Synergistic Effects of Polyphenols and Methylxanthines with Leucine on AMPK/Sirtuin-Mediated Metabolism in Muscle Cells and Adipocytes
PLoS ONE
February 14, 2014
Antje Bruckbauer, Michael B. Zemel
air. Confluent pre-
adipocytes
were induced to differentiate with a standard differentiation medium (
DM2-L1
,
Zen-Bio Inc
., NC). Pre-
adipocytes
were maintained in this differentiation medium for 3 days and subsequently
(15)
Synergistic effects of metformin, resveratrol, and hydroxymethylbutyrate on insulin sensitivity
Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy
February 23, 2013
Michael Bruckbauer, Michael B Bruckbauer
atmosphere. Confluent preadipocytes were induced to differentiate using a standard differentiation medium (
DM2L1
,
Zen-Bio Inc
, Research Triangle Park, NC, USA). The preadipocytes were maintained in this differentiation
(16)
Mechanisms of Adipocytokine-Mediated Trastuzumab Resistance in HER2-Positive Breast Cancer Cell Lines
Curr Pharmacogenomics Person Med
January 17, 2013
Samantha E Griner, Katherine J Wang, Jayashree P Joshi, Rita Nahta
for days 0 through 6 (Fig. (1A)). During days 6 to 9, cells were cultured in Differentiation Medium (
DM-2-L1
;
Zen-Bio
), which contains isobutylmethylxanthine and a PPARy agonist. Differentiation was confirmed
(17)
Hkat, a novel nutritionally regulated transmembrane protein in adipose tissues
Scientific Reports
December 21, 2012
Ren Zhang
preadipocyte medium (Catalog number
PM-1-L1
,
Zenbio
, Research Triangle Park, NC), differentiation medium (
DM-2-L1
) and
adipocyte
maintenance medium (
AM-1-L1
). Briefly,
3T3 L1 cells
was allowed to reach confluence in
(18)
Inositol Pyrophosphates Inhibit Akt Signaling, Thereby Regulating Insulin Sensitivity and Weight Gain
Cell
December 10, 2010
Anutosh Chakraborty et al.
were maintained in preadipocyte media (
PM-1-L1
), differentiated for 3 days with differentiation media (
DM-2-L1
). After 3 days of differentiation, cells were maintained for another 7 days in
adipocyte
maintenance
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3T3-L1 Differentiation Medium, 100ml from Zen-Bio, Inc.
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